Isolation and identification of soil bacteria resistant to the emerging contamination elements

Jian-Hong Lin and N.-C. Lin

Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan, China

r07623022@ntu.edu.tw

Gallium (Ga), indium (In) and thallium (Tl) are regarded as the emerging contamination elements (ECEs) due to the development of the high-tech industries. The potential risk of Ga, In and Tl to human health and environment initiates us to screen soil microorganisms that are tolerant to Ga, In or Tl for future application, including bioremediation. In this study, soil samples collected from Taoyuan (PC and SK2 series) and Yilan (CI series) were used to isolate microorganisms tolerant to Ga, In or Tl. Bacteria tolerant to Ga, In and Tl in each soil sample were enriched in basal salt medium (BSM) supplemented with two different concentrations of Ga, In or Tl by transferring 1/10 volume to fresh medium (also supplemented with the same concentration of the same element) every ten days. As results,we obtained 44 candidate bacterial strains based on their colony morphology and the amount on nutrient agar after enrichment for 30 days. Among those strains, 23, 14, and 29 isolates can tolerate 200mg L-1 Ga, In and Tl, respectively. Additionally, five, four and twelve strains can tolerate only Ga, In and Tl, respectively. Seven isolates can tolerate both Ga and Tl, two isolates can tolerate both In and Tl, but none can tolerate both Ga and In. Interestingly, eight isolates possess ability to tolerate all three elements. Identification and characterization of these strains are undergoing. Further investigation on the resistant mechanisms of each candidate strain shall reveal how they can survive in the presence of high concentration of ECEs and then candidates for future utilization can be identified.

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