Microbial hotspots, defined as small soil volumes with much faster reaction rates and much more intensive interactions in soils, make significant contributions to the biogeochemical cycling. Rhizosphere and root-detritusphere are two typical hotspots which dominate the element transferring between soils and plants. Labile flux maxima of As can be observed both in rice rhizosphere and root-detritusphere areas, specifically in root tips for living root and along the root axis for dead root. However, in contrast to the spatial distribution of As bioavailability, significant depletion of P labile flux was found around both the living and dead roots. The P labile flux decreases by 68–76% in the root-detritusphere area (2–4 mm from the root axis), similar to the spatial patterns of Fe. Decomposition of dead roots might provide channels for O₂ diffusion, supporting the aerobic microsites in deeper soils and leading to the lower kinetics of P exchange process from soils to soil solution. Generally, the in-situ high resolution methods combining DGT-LA-ICPMS and planar optode support a more holistic understanding of biogeochemical processes of elements in the soil hotspots.