Cadmium isn’t the essential element for rice. However, it can enter rice by magnesium or manganese transporter, and accumulates in grains later, which would seriously affect food safety. Diseases caused by Cd include chronic kidney toxicity, ‘itai-itai disease’, cancer and other health problems. As a way to reduce the effective Cd in soil, people implement paddy field water management. However, this way increased the effectiveness of As in the soil. Developing low Cd-accumulation rice cultivars seems to be a better plan. To further research into the key genes of Cd accumulation, we adopted 243 RILs from the Cd-tolerant cultivar TNG67 and the Cd-sensitive cultivar TN1 as material. RADseq was conducted to make a linkage map of the 243 RILs. By comparing the linkage map with the phenotype under Cd stress, we selected 3 potential QTLs, HMA3, CdT1 and CdT2, which are related to Cd-accumulation. In the future, we would confirm the function of the potential QTLs by gene deletion or gene editing. As more Cd-accumulation genes are revealed, it’s possible to make low Cd-accumulation cultivars by QTL pyramiding or gene editing.