Study on Environmental Behavior and Plant Physiological Effects of Antibiotics in Soil-Plant System

Wu Jiana and Zhang Yuana

a School of Environmental Science and Engineering, Suzhou University of Science and Technology, China

976429048@qq.com

Manure application is a common agricultural behavior that not only adds nutrients to soils for plant growth, but also introduces antibiotics and antibiotic resistance genes (ARGs), posing a potential risk to human health. To study the spread mechanisms of antibiotics and ARGs in soil-plant system, especially their interaction, degradation and transport patterns from soil to plants, a greenhouse pot experiment is conducting. Six typical plants, including three native plants such as crabgrass, barnyard grass and green bristlegrass, and three common crops of lettuce, cabbage and corn, all of which are planted in soil contaminated with antibiotics from farms. The HPLC-MS-MS and High-throughput sequencing to be used to determine the concentration of antibiotics and the abundance and expression of ARGs in soil and plants, to indicate the degradation and distribution of antibiotic and ARGs in different soil-plant systems and the relative abundance and expression levels of ARGs in different plants after harvesting. This study expects to discuss the interactive behaviors among different soil-antibiotics/ARGs-plants, and to identify the differences in migration and transformation level of antibiotics and ARGs between the long-term and short-term antibiotic-contaminated soil-plant systems, so as to derive the risks of antibiotics and resistance genes to plants. These findings may shed light on the mechanisms underlying the spread and fate of antibiotics and ARGs in manured soil and clue for phytoremediation of soil contaminated with antibiotics.

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