The use of fast-growing trees producing a high quantity of biomass can bring significant practical and economic benefits to the reclamation of marginal lands. The present study aims to identify new tree species to offer a wider range of trees useful for phytomanagement practices. We implemented three experimental sites in France of 1 ha each (Thann, Carrières-sous-Poissy and Leforest) contaminated by different potentially toxic elements (PTE) with a total of 38 different tree species. The 3 sites were divided into 8 different plots. In order to improve plant remediative capacities, 4 of the 8 plots were inoculated with a commercial mycorrhizal inoculum (Rhizophagus irregulare DAOM 197198 strain). In this study, microbial community structure on rhyzobial soils were analysed using metabarcoding approach (IlluminaMiSeq). Both 16S and ITs primers were used to amplified bacteria and fungi present on each of the three sites and for each of the 38 tree species. Fungal analyse results show that fungi communities are specific of the plant species. For instance, Salix aquatica grandis (a common species for the three sites), previously demonstrated to accumulate high concentration of TE in aboveground biomass, showed no significant difference depending on the site. However, significant dissimilarities were exhibited for Pterocarya stenoptera and Ostrya carpinofolia (the other 2 common species for the three sites), trees with phytostabilisation capacity. Moreover, this study showed that some tree species contribute to change the fungal community in soil if compared to the control soil. On this regard, Carrières control soil were composed by 30% of Mortierellales, 40% of Hypocreales and 30% of 11 other fungi, while, e.g., rhyzobial soil of Salix aquatica grandis presented a very different fungal composition, with a higher proportion of Agaricales (60%). In opposition, bacterial communities exhibited no significant difference depending the tree species neither compared to the control soil. Furthermore, bacterial composition was different from one site to another, demonstrating that soil physic-chemical characteristics play a crucial role in microbial community. Additional details about microbial community structure will be further discussed in the presentation.