When pollutants enter the soil, they will adversely affect soil organisms, leading to a decline in soil ecosystem function. Microbes are sensitive to heavy metal stress, so microbial indicators are often used to assess soil pollution levels. Soil antimony pollution is getting more and more serious with increasing industrial use. In order to explore the effects of different concentrations and different forms of antimony on microbial activity and community diversity in different types of soil, four different types of soils with different pH were selected for testing, different concentrations and different forms of antimony were added to the soil. The microbial indicators such as effective antimony content, soil respiration intensity, enzyme activity and nitrification rate will be measured periodically. The soil microbial community structure and function will be determined by high-throughput sequencing and gene chip. The purpose of this study is to explore the relationship between microbial activity, microbial community diversity and antimony concentration under different soil types and different degrees of antimony pollution stress. It can provide a reference for assessing the risk of soil antimony pollution.