Earthworms, as one of the most widely distributed invertebrates in soil, which make up the largest amount of animal biomass in soil, are critical to ecosystem services and functions. Earthworms are usually considered as biological indicators to predict the bioavailability of Sb in soil and an important component of ecological risk assessments. Avoidance tests can be used as a kind of quick and cost-effective method for assessing contaminants in soils. Laboratory experiments of earthworms with series of concentrations of antimony (Sb) spiked agricultural soil were conducted in a climatron to study the chronic toxicity and avoidance response of Sb on Eisenia Fetida. The mortality rates and growth inhibition of 28d culture increased significantly when the level of Sb in the soils above 500 mg/kg. The bioaccumulation of Sb by earthworms increased with the increasing concentration of Sb in the soils, and there were no statistically significant increases in the level of Sb of the soils under 800 mg/kg. For Eisenia Fetida, significant differences(P>0.05) were found in the distribution of the worms between the untreated sides and the sides with treated soil, the avoidance response by worms decreased with the increasing concentration of Sb addition. The growth inhibition rates and bioaccumulation were both significantly correlated with the exposure dose, and might be used as sensitive indicator for ecological risk assessment of soils. This study provided toxicological data that can contribute to the development of Eco-SSLs for Sb, as well.