Arsenic (As) causes number of serious diseases. Rice is the major food for arsenic (As) intake in human, since it efficiently accumulates As much more than other cereal crops. Identification of genes and the molecular mechanisms for As transport and detoxification is crucial step to develop lower As rice. Hear we identified 25 rice genes participating As resistance using yeast expression system with rice cDNA library generated from mRNA of As-treated rice roots. Among of them, AsRT1 (a zinc finger type transcription factor) exhibited the most dramatically improved As resistance in yeast cells and inhibited As accumulation through the regulation of many different mechanisms, such as As efflux, As uptake and ubiquitination in yeast. The loss of functional mutants of Arabidopsis and rice AsRT1 and AsRT2 expressing phloem in shoot less accumulated As in seeds. The calli generated from atasrt1atasrt2 exhibited enhanced As resistance and reduced As accumulation compared to those of WT. The results from As feeding assay with rosette leaves and stem of Arabidopsis and rice showed that the Arabidopsis and rice mutants had reduced As transport activity to neighbor tissues through the phloem pathway. Our results suggest that rice and Arabidopsis AsRTs are the transcription factors to regulate As translocation into seeds through the phloem pathway.