Changes of mycorrhizal fungal communities during phytoremediation of red mud and heavy metal contaminated soil by Arundo donax

Gaozhong Pu

Guangxi Key Laboratory of Plant Conservation and Restoration Ecology in Karst Terrain,Guangxi Institue of Botany,Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences, China

pukouchy@hotmail.com

Mycorrhizal fungi may show more potential in phytoremediation than rhizosphere microbes by providing nutrients to plants and enhancing metal bioavailability in the rhizosphere of plants by producing metabolites. However, little is known about the effects soil pollution type on mycorrhizal fungal communities. In present study, the diversity and composition of mycorrhizal fungal from Arundo donax, a good candidates to phytoremediation, which grown in different soil types (including metal pollution soil, red mud and un-pollution soil) were compared using the Illumina MiSeq sequencing technique. The results showed that the heavy metal polluted soil had significant higher mycorrhizal fungi alpha diversity than those from unpolluted and red mud area. Non-metric multidimensional scaling (NMDS) analysis showed significant differences among different soil types. At genus level, Marasmiellus sp.(81.16%)was enriched in the unpolluted soil, Zopfiella_marina (56.52%) in heavy metal soil and an unclassified species (Hypocreales) (93.17%). This effort to profile the background diversity may set the first stage for the remediation of contaminated soil by plant-mycorrhizal fungal combinations.

Keywords: Mycorrhizal fungi, MiSeq sequencing, red mud, phytostabilisation, Arundo donax

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